Comparative study of performances of UHPLC-MS/MS and HPLC/UV methods for analysis of ziprasidone and its main impurities

Abstract Ziprasidone is the second generation antipsychotic drug with unique multipotent G-protein-coupled (GPCR) receptor binding profile. Since ziprasidone a highly lipophilic and unstable compound, development of efficient method for concurrent assay its main impurities was very challenging task. The UHPLC-MS/MS that we developed simultaneous determination (BITP, Chloroethyl-chloroindolinone, Zip-oxide, Zip-dimer, Zip-BIT) compared some other related HPLC-UV methods our own authorship. An increase mobile phase pH value from 2.5 to 4.7 units in examined analytical influenced elution order investigated compounds. It found out more selective sensitive than earlier method. Similar method, linear correlation coefficient ( r ) above 0.99 all analysed compounds, but negligibly lower precision accuracy. Finally, shorter analysis time, smaller column size reduction solvent consumption, assumed as greener purity assay. After transfer UHPLC-DAD system, suitability routine control confirmed based on retained good selectivity, resolution short time.